5MZN

Helicase Sen1

5MZN の概要

• エントリーDOI: 10.2210/pdb5mzn/pdb
• 分子名称: Helicase SEN1,Helicase SEN1, ADENOSINE-5'-DIPHOSPHATE, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: yeast helicase domain sen1, hydrolase
• 由来する生物種: Saccharomyces cerevisiae S288c; 詳細
• 細胞内の位置: Nucleus : Q00416
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90372.83

構造登録者

Leonaite, B.,Basquin, J.,Conti, E. (登録日: 2017-02-01, 公開日: 2017-04-19, 最終更新日: 2025-10-01)

主引用文献

Leonaite, B.,Han, Z.,Basquin, J.,Bonneau, F.,Libri, D.,Porrua, O.,Conti, E.
Sen1 has unique structural features grafted on the architecture of the Upf1-like helicase family.
EMBO J., 36:1590-1604, 2017

PubMed Abstract: The superfamily 1B (SF1B) helicase Sen1 is an essential protein that plays a key role in the termination of non-coding transcription in yeast. Here, we identified the ~90 kDa helicase core of Sen1 as sufficient for transcription termination and determined the corresponding structure at 1.8 Å resolution. In addition to the catalytic and auxiliary subdomains characteristic of the SF1B family, Sen1 has a distinct and evolutionarily conserved structural feature that "braces" the helicase core. Comparative structural analyses indicate that the "brace" is essential in shaping a favorable conformation for RNA binding and unwinding. We also show that subdomain 1C (the "prong") is an essential element for 5'-3' unwinding and for Sen1-mediated transcription termination Finally, yeast Sen1 mutant proteins mimicking the disease forms of the human orthologue, senataxin, show lower capacity of RNA unwinding and impairment of transcription termination The combined biochemical and structural data thus provide a molecular model for the specificity of Sen1 in transcription termination and more generally for the unwinding mechanism of 5'-3' helicases.

PubMed: 28408439
DOI: 10.15252/embj.201696174

実験手法

X-RAY DIFFRACTION (1.787 Å)