5MYJ

Structure of 70S ribosome from Lactococcus lactis

これはPDB形式変換不可エントリーです。

5MYJ の概要

• エントリーDOI: 10.2210/pdb5myj/pdb
• EMDBエントリー: 3581
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (51 entities in total)
• 機能のキーワード: ribosome, 70s, lactoccocus lactis, cryo-em
• 由来する生物種: Lactococcus lactis subsp. cremoris MG1363; 詳細
• 細胞内の位置: Cytoplasm : A2RIX0
• タンパク質・核酸の鎖数: 51
• 化学式量合計: 2115462.30

構造登録者

Franken, L.E.,Oostergetel, G.T.,Pijning, T.,Puri, P.,Boekema, E.J.,Poolman, B.,Guskov, A. (登録日: 2017-01-26, 公開日: 2017-10-11, 最終更新日: 2024-05-15)

主引用文献

Franken, L.E.,Oostergetel, G.T.,Pijning, T.,Puri, P.,Arkhipova, V.,Boekema, E.J.,Poolman, B.,Guskov, A.
A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.
Nat Commun, 8:722-722, 2017

PubMed Abstract: Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes from Lactococcus lactis and a dimerization mechanism involving a single protein: HPF. The N-terminal domain of HPF binds at the same site as HPF in Escherichia coli 100S ribosomes. Contrary to ribosome modulation factor, the C-terminal domain of HPF binds exactly at the dimer interface. Furthermore, ribosomes from Lactococcus lactis do not undergo conformational changes in the 30S head domains upon binding of HPF, and the Shine-Dalgarno sequence and mRNA entrance tunnel remain accessible. Ribosome activity is blocked by HPF due to the inhibition of mRNA recognition by the platform binding center. Phylogenetic analysis of HPF proteins suggests that HPF-mediated dimerization is a widespread mechanism of ribosome hibernation in bacteria.When bacteria enter the stationary growth phase, protein translation is suppressed via the dimerization of 70S ribosomes into inactive complexes. Here the authors provide a structural basis for how the dual domain hibernation promotion factor promotes ribosome dimerization and hibernation in bacteria.

PubMed: 28959045
DOI: 10.1038/s41467-017-00718-x

実験手法

ELECTRON MICROSCOPY (5.6 Å)