5MXG

Photorhabdus asymbiotica lectin (PHL) in complex with blood group H trisaccharide

Summary for 5MXG

• Entry DOI: 10.2210/pdb5mxg/pdb
• Related PRD ID: PRD_900036
• Descriptor: Photorhabdus asymbiotica lectin PHL, alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SODIUM ION, ... (4 entities in total)
• Functional Keywords: lectin, seven-bladed beta-propeller, bgh trisaccharide, sugar binding protein
• Biological source: Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77)
• Total number of polymer chains: 1
• Total formula weight: 41824.56

Authors

Jancarikova, G.,Houser, J.,Demo, G.,Wimmerova, M. (deposition date: 2017-01-23, release date: 2017-08-09, Last modification date: 2024-01-17)

Primary citation

Jancarikova, G.,Houser, J.,Dobes, P.,Demo, G.,Hyrsl, P.,Wimmerova, M.
Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity.
PLoS Pathog., 13:e1006564-e1006564, 2017

PubMed Abstract: Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. Recombinant PHL exhibited high affinity for fucosylated carbohydrates and the unusual disaccharide 3,6-O-Me2-Glcβ1-4(2,3-O-Me2)Rhaα-O-(p-C6H4)-OCH2CH2NH2 from Mycobacterium leprae. Based on its crystal structure, PHL forms a seven-bladed β-propeller assembling into a homo-dimer with an inter-subunit disulfide bridge. Investigating complexes with different ligands revealed the existence of two sets of binding sites per monomer-the first type prefers l-fucose and its derivatives, whereas the second type can bind d-galactose. Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. PHL was shown to interact with all types of red blood cells and insect haemocytes. Interestingly, PHL inhibited the production of reactive oxygen species induced by zymosan A in human blood and antimicrobial activity both in human blood, serum and insect haemolymph. Concurrently, PHL increased the constitutive level of oxidants in the blood and induced melanisation in haemolymph. Our results suggest that PHL might play a crucial role in the interaction of P. asymbiotica with both human and insect hosts.

PubMed: 28806750
DOI: 10.1371/journal.ppat.1006564

Experimental method

X-RAY DIFFRACTION (2.2 Å)