5MWF

Human Jagged2 C2-EGF2

Summary for 5MWF

• Entry DOI: 10.2210/pdb5mwf/pdb
• Descriptor: Protein jagged-2, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: c2, egf, notch, signaling protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 6
• Total formula weight: 199982.27

Authors

Suckling, R.J.,Handford, P.A.,Lea, S.M. (deposition date: 2017-01-18, release date: 2017-06-14, Last modification date: 2024-10-23)

Primary citation

Suckling, R.J.,Korona, B.,Whiteman, P.,Chillakuri, C.,Holt, L.,Handford, P.A.,Lea, S.M.
Structural and functional dissection of the interplay between lipid and Notch binding by human Notch ligands.
EMBO J., 36:2204-2215, 2017

PubMed Abstract: Recent data have expanded our understanding of Notch signalling by identifying a C2 domain at the N-terminus of Notch ligands, which has both lipid- and receptor-binding properties. We present novel structures of human ligands Jagged2 and Delta-like4 and human Notch2, together with functional assays, which suggest that ligand-mediated coupling of membrane recognition and Notch binding is likely to be critical in establishing the optimal context for Notch signalling. Comparisons between the Jagged and Delta family show a huge diversity in the structures of the loops at the apex of the C2 domain implicated in membrane recognition and Jagged1 missense mutations, which affect these loops and are associated with extrahepatic biliary atresia, lead to a loss of membrane recognition, but do not alter Notch binding. Taken together, these data suggest that C2 domain binding to membranes is an important element in tuning ligand-dependent Notch signalling in different physiological contexts.

PubMed: 28572448
DOI: 10.15252/embj.201796632

Experimental method

X-RAY DIFFRACTION (2.8 Å)