5MVU

Crystal structure of an A-DNA dodecamer containing 5-formylcytosine in 3 consecutive CpG steps

5MVU の概要

• エントリーDOI: 10.2210/pdb5mvu/pdb
• 分子名称: DNA (2 entities in total)
• 機能のキーワード: a-dna, dna, 5-formylcytosine, cpg
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7494.84

構造登録者

Hardwick, J.S.,Ptchelkine, D.,Phillips, S.E.V.,Brown, T. (登録日: 2017-01-17, 公開日: 2017-05-10, 最終更新日: 2024-01-17)

主引用文献

Hardwick, J.S.,Ptchelkine, D.,El-Sagheer, A.H.,Tear, I.,Singleton, D.,Phillips, S.E.V.,Lane, A.N.,Brown, T.
5-Formylcytosine does not change the global structure of DNA.
Nat. Struct. Mol. Biol., 24:544-552, 2017

PubMed Abstract: The mechanism by which the recently identified DNA modification 5-formylcytosine (C) is recognized by epigenetic writer and reader proteins is not known. Recently, an unusual DNA structure, F-DNA, has been proposed as the basis for enzyme recognition of clusters of C. We used NMR and X-ray crystallography to compare several modified DNA duplexes with unmodified analogs and found that in the crystal state the duplexes all belong to the A family, whereas in solution they are all members of the B family. We found that, contrary to previous findings, C does not significantly affect the structure of DNA, although there are modest local differences at the modification sites. Hence, global conformation changes are unlikely to account for the recognition of this modified base, and our structural data favor a mechanism that operates at base-pair resolution for the recognition of C by epigenome-modifying enzymes.

PubMed: 28504696
DOI: 10.1038/nsmb.3411

実験手法

X-RAY DIFFRACTION (2.3 Å)