5MSH

Cowpea mosaic virus top component (CPMV-T) - naturally occurring empty particles

5MSH の概要

• エントリーDOI: 10.2210/pdb5msh/pdb
• EMDBエントリー: 3565
• 分子名称: Cowpea mosaic virus small subunit, Cowpea mosaic virus large subunit (2 entities in total)
• 機能のキーワード: cpmv-m, plant virus, picornavirales, virus
• 由来する生物種: Cowpea mosaic virus (CPMV); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61820.00

構造登録者

Hesketh, E.L.,Meshcheriakova, Y.,Thompson, R.F.,Lomonossoff, G.P.,Ranson, N.A. (登録日: 2017-01-04, 公開日: 2017-07-19, 最終更新日: 2024-05-15)

主引用文献

Hesketh, E.L.,Meshcheriakova, Y.,Thompson, R.F.,Lomonossoff, G.P.,Ranson, N.A.
The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.
Sci Rep, 7:539-539, 2017

PubMed Abstract: Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.

PubMed: 28373698
DOI: 10.1038/s41598-017-00533-w

実験手法

ELECTRON MICROSCOPY (4.25 Å)