5MS1
Cowpea mosaic virus top component (CPMV-T) - naturally occurring empty particles
Summary for 5MS1
| Entry DOI | 10.2210/pdb5ms1/pdb |
| EMDB information | 3562 |
| Descriptor | Small subunit of cowpea mosaic virus, Large subunit of Cowpea music virus (2 entities in total) |
| Functional Keywords | cpmv-t, plant virus, picornavirales, virus |
| Biological source | Cowpea mosaic virus (CPMV) More |
| Total number of polymer chains | 2 |
| Total formula weight | 64657.34 |
| Authors | Hesketh, E.L.,Meshcheriakova, Y.,Thompson, R.F.,Lomonossoff, G.P.,Ranson, N.A. (deposition date: 2016-12-29, release date: 2017-07-19, Last modification date: 2024-05-15) |
| Primary citation | Hesketh, E.L.,Meshcheriakova, Y.,Thompson, R.F.,Lomonossoff, G.P.,Ranson, N.A. The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Sci Rep, 7:539-539, 2017 Cited by PubMed Abstract: Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool. PubMed: 28373698DOI: 10.1038/s41598-017-00533-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.25 Å) |
Structure validation
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