5MQX

NMR solution structure of macro domain from Venezuelan equine encephalitis virus(VEEV) in complex with ADP-ribose

5MQX の概要

• エントリーDOI: 10.2210/pdb5mqx/pdb
• NMR情報: BMRB: 26753
• 分子名称: Non-structural protein3, ADENOSINE-5-DIPHOSPHORIBOSE (2 entities in total)
• 機能のキーワード: macro domain, venezuelan equine encephalitis virus, alphavirus, adp-ribose, viral protein, non-structural protein 3
• 由来する生物種: Venezuelan equine encephalitis virus (strain P676) (VEEV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18663.81

構造登録者

Makrynitsa, G.I.,Ntonti, D.,Marousis, K.D.,Matsoukas, M.T.,Papageorgiou, N.,Coutard, B.,Bentrop, D.,Spyroulias, G.A. (登録日: 2016-12-21, 公開日: 2018-07-04, 最終更新日: 2024-06-19)

主引用文献

Makrynitsa, G.I.,Ntonti, D.,Marousis, K.D.,Birkou, M.,Matsoukas, M.T.,Asami, S.,Bentrop, D.,Papageorgiou, N.,Canard, B.,Coutard, B.,Spyroulias, G.A.
Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose.
J.Struct.Biol., 206:119-127, 2019

PubMed Abstract: Venezuelan equine encephalitis virus (VEEV) is a new world alphavirus which can be involved in several central nervous system disorders such as encephalitis and meningitis. The VEEV genome codes for 4 non-structural proteins (nsP), of which nsP3 contains a Macro domain. Macro domains (MD) can be found as stand-alone proteins or embedded within larger proteins in viruses, bacteria and eukaryotes. Their most common feature is the binding of ADP-ribose (ADPr), while several macro domains act as ribosylation writers, erasers or readers. Alphavirus MD erase ribosylation but their precise contribution in viral replication is still under investigation. NMR-driven titration experiments of ADPr in solution with the VEEV macro domain (in apo- and complex state) show that it adopts a suitable conformation for ADPr binding. Specific experiments indicate that the flexibility of the loops β5-α3 and α3-β6 is critical for formation of the complex and assists a wrapping mechanism for ADPr binding. Furthermore, along with this sequence of events, the VEEV MD undergoes a conformational exchange process between the apo state and a low-populated "dark" conformational state.

PubMed: 30825649
DOI: 10.1016/j.jsb.2019.02.008

実験手法

SOLUTION NMR