5MOU

NMR spatial structure of scorpion alpha-like toxin BeM9

5MOU の概要

• エントリーDOI: 10.2210/pdb5mou/pdb
• NMR情報: BMRB: 34078
• 分子名称: Alpha-mammal toxin BeM9 (1 entity in total)
• 機能のキーワード: protein neurotoxin, protein
• 由来する生物種: Mesobuthus eupeus (Lesser Asian scorpion)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7351.35

構造登録者

Mineev, K.S.,Kuldushev, N.A.,Berkut, A.A.,Grishin, E.V.,Vassilevski, A.A.,Arseniev, A.S. (登録日: 2016-12-14, 公開日: 2018-01-17, 最終更新日: 2023-06-14)

主引用文献

Kuldyushev, N.A.,Mineev, K.S.,Berkut, A.A.,Peigneur, S.,Arseniev, A.S.,Tytgat, J.,Grishin, E.V.,Vassilevski, A.A.
Refined structure of BeM9 reveals arginine hand, an overlooked structural motif in scorpion toxins affecting sodium channels.
Proteins, 86:1117-1122, 2018

PubMed Abstract: Sodium channel alpha-toxins from scorpion venom (α-NaTx) inhibit the inactivation of voltage-gated sodium channels. We used solution NMR to investigate the structure of BeM9 toxin from Mesobuthus eupeus scorpion, a prototype α-NaTx classified as an "α-like" toxin due to its wide spectrum of activity on insect and mammalian channels. We identified a new motif that we named "arginine hand," whereby arginine side chain forms several hydrogen bonds with main chain atoms. The arginine hand was found in the "specificity module," a part of the molecule that dictates toxin selectivity; and just single arginine-to-lysine point mutation drastically changed BeM9 selectivity profile.

PubMed: 30007037
DOI: 10.1002/prot.25583

実験手法

SOLUTION NMR