5MMU

NMR solution structure of the major apple allergen Mal d 1

5MMU の概要

• エントリーDOI: 10.2210/pdb5mmu/pdb
• NMR情報: BMRB: 25968
• 分子名称: Major allergen Mal d 1 (1 entity in total)
• 機能のキーワード: pr-10 protein apple allergen, allergen
• 由来する生物種: Malus domestica (Apple)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17543.69

構造登録者

Ahammer, L.,Grutsch, S.,Kamenik, A.S.,Liedl, K.R.,Tollinger, M. (登録日: 2016-12-12, 公開日: 2017-02-15, 最終更新日: 2024-05-15)

主引用文献

Ahammer, L.,Grutsch, S.,Kamenik, A.S.,Liedl, K.R.,Tollinger, M.
Structure of the Major Apple Allergen Mal d 1.
J. Agric. Food Chem., 65:1606-1612, 2017

PubMed Abstract: More than 70% of birch pollen-allergic patients develop allergic cross-reactions to the major allergen found in apple fruits (Malus domestica), the 17.5 kDa protein Mal d 1. Allergic reactions against this protein result from initial sensitization to the major allergen from birch pollen, Bet v 1. Immunologic cross-reactivity of Bet v 1-specific IgE antibodies with Mal d 1 after apple consumption can subsequently provoke severe oral allergic syndromes. This study presents the three-dimensional NMR solution structure of Mal d 1 (isoform Mal d 1.0101, initially cloned from 'Granny Smith' apples). This protein is composed of a seven-stranded antiparallel β-sheet and three α-helices that form a large internal cavity, similar to Bet v 1 and other cross-reactive food allergens. The Mal d 1 structure provides the basis for elucidating the details of allergic cross-reactivity between birch pollen and apple allergens on a molecular level.

PubMed: 28161953
DOI: 10.1021/acs.jafc.6b05752

実験手法

SOLUTION NMR