5MM2

nora virus structure

5MM2 の概要

• エントリーDOI: 10.2210/pdb5mm2/pdb
• EMDBエントリー: 3528
• 分子名称: capsid protein VP4C, capsid protein VP4B, Capsid protein VP4A (3 entities in total)
• 機能のキーワード: nora virus, cryo-em, single particle analysis, capsid, virus
• 由来する生物種: Nora virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 102444.91

構造登録者

Laurinmaki, P.,Shakeel, S.,Ekstrom, J.-O.,Butcher, S.J. (登録日: 2016-12-08, 公開日: 2017-12-20, 最終更新日: 2024-05-22)

主引用文献

Laurinmaki, P.,Shakeel, S.,Ekstrom, J.O.,Mohammadi, P.,Hultmark, D.,Butcher, S.J.
Structure of Nora virus at 2.7 angstrom resolution and implications for receptor binding, capsid stability and taxonomy.
Sci Rep, 10:19675-19675, 2020

PubMed Abstract: Nora virus, a virus of Drosophila, encapsidates one of the largest single-stranded RNA virus genomes known. Its taxonomic affinity is uncertain as it has a picornavirus-like cassette of enzymes for virus replication, but the capsid structure was at the time for genome publication unknown. By solving the structure of the virus, and through sequence comparison, we clear up this taxonomic ambiguity in the invertebrate RNA virosphere. Despite the lack of detectable similarity in the amino acid sequences, the 2.7 Å resolution cryoEM map showed Nora virus to have T = 1 symmetry with the characteristic capsid protein β-barrels found in all the viruses in the Picornavirales order. Strikingly, α-helical bundles formed from the extended C-termini of capsid protein VP4B and VP4C protrude from the capsid surface. They are similar to signalling molecule folds and implicated in virus entry. Unlike other viruses of Picornavirales, no intra-pentamer stabilizing annulus was seen, instead the intra-pentamer stability comes from the interaction of VP4C and VP4B N-termini. Finally, intertwining of the N-termini of two-fold symmetry-related VP4A capsid proteins and RNA, provides inter-pentamer stability. Based on its distinct structural elements and the genetic distance to other picorna-like viruses we propose that Nora virus, and a small group of related viruses, should have its own family within the order Picornavirales.

PubMed: 33184473
DOI: 10.1038/s41598-020-76613-1

実験手法

ELECTRON MICROSCOPY (2.7 Å)