5MKK

Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP

5MKK の概要

• エントリーDOI: 10.2210/pdb5mkk/pdb
• 分子名称: Multidrug resistance ABC transporter ATP-binding and permease protein, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: abc transporter, multidrug resistance, antigenic peptide transporter tap, lipid transport, transport protein
• 由来する生物種: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 133790.44

構造登録者

Noell, A.,Thomas, C.,Tomasiak, T.M.,Olieric, V.,Wang, M.,Diederichs, K.,Stroud, R.M.,Pos, K.M.,Tampe, R. (登録日: 2016-12-05, 公開日: 2017-01-18, 最終更新日: 2024-05-08)

主引用文献

Noll, A.,Thomas, C.,Herbring, V.,Zollmann, T.,Barth, K.,Mehdipour, A.R.,Tomasiak, T.M.,Bruchert, S.,Joseph, B.,Abele, R.,Olieric, V.,Wang, M.,Diederichs, K.,Hummer, G.,Stroud, R.M.,Pos, K.M.,Tampe, R.
Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Proc. Natl. Acad. Sci. U.S.A., 114:E438-E447, 2017

PubMed Abstract: ABC transporters form one of the largest protein superfamilies in all domains of life, catalyzing the movement of diverse substrates across membranes. In this key position, ABC transporters can mediate multidrug resistance in cancer therapy and their dysfunction is linked to various diseases. Here, we describe the 2.7-Å X-ray structure of heterodimeric Thermus thermophilus multidrug resistance proteins A and B (TmrAB), which not only shares structural homology with the antigen translocation complex TAP, but is also able to restore antigen processing in human TAP-deficient cells. TmrAB exhibits a broad peptide specificity and can concentrate substrates several thousandfold, using only one single active ATP-binding site. In our structure, TmrAB adopts an asymmetric inward-facing state, and we show that the C-terminal helices, arranged in a zipper-like fashion, play a crucial role in guiding the conformational changes associated with substrate transport. In conclusion, TmrAB can be regarded as a model system for asymmetric ABC exporters in general, and for TAP in particular.

PubMed: 28069938
DOI: 10.1073/pnas.1620009114

実験手法

X-RAY DIFFRACTION (2.7 Å)