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5MKC

Crystal structure of the RrgA Jo.In complex

5MKC の概要
エントリーDOI10.2210/pdb5mkc/pdb
分子名称Cell wall surface anchor family protein (Jo),Cell wall surface anchor family protein (In), SULFATE ION, NICKEL (II) ION, ... (5 entities in total)
機能のキーワードbacteria, peptidoglycan, pilus adhesin, cross-links, folding, post-translational, isopeptide bond, biotechnology, cell adhesion
由来する生物種Streptococcus pneumoniae
詳細
タンパク質・核酸の鎖数6
化学式量合計158575.82
構造登録者
Bonnet, J.,Cartannaz, J.,Tourcier, G.,Contreras-Martel, C.,Kleman, J.P.,Fenel, D.,Schoehn, G.,Morlot, C.,Vernet, T.,Di Guilmi, A.M. (登録日: 2016-12-03, 公開日: 2017-03-15, 最終更新日: 2024-10-16)
主引用文献Bonnet, J.,Cartannaz, J.,Tourcier, G.,Contreras-Martel, C.,Kleman, J.P.,Morlot, C.,Vernet, T.,Di Guilmi, A.M.
Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.
Sci Rep, 7:43564-43564, 2017
Cited by
PubMed Abstract: Unusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 Å. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.
PubMed: 28252635
DOI: 10.1038/srep43564
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.04 Å)
構造検証レポート
Validation report summary of 5mkc
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-30に公開中

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