5MJD

metNgb under oxygen at 80 bar

Summary for 5MJD

• Entry DOI: 10.2210/pdb5mjd/pdb
• Related: 4O4T 5MJC
• Descriptor: Neuroglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (5 entities in total)
• Functional Keywords: oxygen complex storage cavity, transport protein
• Biological source: Mus musculus (House Mouse)
• Total number of polymer chains: 1
• Total formula weight: 17379.42

Authors

Prange, T.,Colloc'h, N.,Carpentier, P. (deposition date: 2016-11-30, release date: 2017-12-20, Last modification date: 2024-01-17)

Primary citation

Ardiccioni, C.,Arcovito, A.,Della Longa, S.,van der Linden, P.,Bourgeois, D.,Weik, M.,Montemiglio, L.C.,Savino, C.,Avella, G.,Exertier, C.,Carpentier, P.,Prange, T.,Brunori, M.,Colloc'h, N.,Vallone, B.
Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.
Iucrj, 6:832-842, 2019

PubMed Abstract: A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV-visible microspectroscopy , solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15-40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.

PubMed: 31576217
DOI: 10.1107/S2052252519008157

Experimental method

X-RAY DIFFRACTION (1.7 Å)