5MJ2

Extracellular domain of human CD83 - rhombohedral crystal form after UV-RIP (S-SAD data)

5MJ2 の概要

• エントリーDOI: 10.2210/pdb5mj2/pdb
• 関連するPDBエントリー: 5MIX 5MJ0 5MJ1
• 分子名称: CD83 antigen, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: dendritic cell, receptor, immunoglobulin, immune system
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: Q01151
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12873.10

構造登録者

Klingl, S.,Egerer-Sieber, C.,Schmid, B.,Weiler, S.,Muller, Y.A. (登録日: 2016-11-29, 公開日: 2017-03-29, 最終更新日: 2018-03-07)

主引用文献

Heilingloh, C.S.,Klingl, S.,Egerer-Sieber, C.,Schmid, B.,Weiler, S.,Muhl-Zurbes, P.,Hofmann, J.,Stump, J.D.,Sticht, H.,Kummer, M.,Steinkasserer, A.,Muller, Y.A.
Crystal Structure of the Extracellular Domain of the Human Dendritic Cell Surface Marker CD83.
J. Mol. Biol., 429:1227-1243, 2017

PubMed Abstract: CD83 is a type-I membrane protein and an efficient marker for identifying mature dendritic cells. Whereas membrane-bound, full-length CD83 co-stimulates the immune system, a soluble variant (sCD83), consisting of the extracellular domain only, displays strong immune-suppressive activities. Besides a prediction that sCD83 adopts a V-set Ig-like fold, however, little is known about the molecular architecture of CD83 and the mechanism by which CD83 exerts its function on dendritic cells and additional immune cells. Here, we report the crystal structure of human sCD83 up to a resolution of 1.7Å solved in three different crystal forms. Interestingly, β-strands C', C″, and D that are typical for V-set Ig-domains could not be traced in sCD83. Mass spectrometry analyses, limited proteolysis experiments, and bioinformatics studies show that the corresponding segment displays enhanced main-chain accessibility, extraordinary low sequence conservation, and a predicted high disorder propensity. Chimeric proteins with amino acid swaps in this segment show unaltered immune-suppressive activities in a TNF-α assay when compared to wild-type sCD83. This strongly indicates that this segment does not participate in the biological activity of CD83. The crystal structure of CD83 shows the recurrent formation of dimers and trimers in the various crystal forms and reveals strong structural similarities between sCD83 and B7 family members and CD48, a signaling lymphocyte activation molecule family member. This suggests that CD83 exerts its immunological activity by mixed homotypic and heterotypic interactions as typically observed for proteins present in the immunological synapse.

PubMed: 28315353
DOI: 10.1016/j.jmb.2017.03.009

実験手法

X-RAY DIFFRACTION (1.98 Å)