5MIP

Crystal Structure of Lactococcus lactis Thioredoxin Reductase Exposed to Visible Light (30 min)

5MIP の概要

• エントリーDOI: 10.2210/pdb5mip/pdb
• 分子名称: Thioredoxin reductase, FLAVIN-ADENINE DINUCLEOTIDE, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (6 entities in total)
• 機能のキーワード: thioredoxin reductase, photosensitivity, reactive oxygen species, fad si-face open space, oxygen pocket, fad-nadp+ complex, fo-fr conformations, oxidoreductase
• 由来する生物種: Lactococcus lactis subsp. cremoris
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37959.93

構造登録者

Skjoldager, N.,Bang, M.B.,Svensson, B.,Hagglund, P.,Harris, P. (登録日: 2016-11-29, 公開日: 2017-04-12, 最終更新日: 2024-11-20)

主引用文献

Skjoldager, N.,Blanner Bang, M.,Rykr, M.,Bjornberg, O.,Davies, M.J.,Svensson, B.,Harris, P.,Hagglund, P.
The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.
Sci Rep, 7:46282-46282, 2017

PubMed Abstract: The NADPH-dependent homodimeric flavoenzyme thioredoxin reductase (TrxR) provides reducing equivalents to thioredoxin, a key regulator of various cellular redox processes. Crystal structures of photo-inactivated thioredoxin reductase (TrxR) from the Gram-positive bacterium Lactococcus lactis have been determined. These structures reveal novel molecular features that provide further insight into the mechanisms behind the sensitivity of this enzyme toward visible light. We propose that a pocket on the si-face of the isoalloxazine ring accommodates oxygen that reacts with photo-excited FAD generating superoxide and a flavin radical that oxidize the isoalloxazine ring C7α methyl group and a nearby tyrosine residue. This tyrosine and key residues surrounding the oxygen pocket are conserved in enzymes from related bacteria, including pathogens such as Staphylococcus aureus. Photo-sensitivity may thus be a widespread feature among bacterial TrxR with the described characteristics, which affords applications in clinical photo-therapy of drug-resistant bacteria.

PubMed: 28397795
DOI: 10.1038/srep46282

実験手法

X-RAY DIFFRACTION (2 Å)