5MHZ

The study of the X-ray induced enzymatic reduction of molecular oxygen to water for laccase from Steccherinum murashkinskyi.The 8-th structure of the series with total exposition time 213 min.

5MHZ の概要

• エントリーDOI: 10.2210/pdb5mhz/pdb
• 分子名称: Laccase 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION, ... (7 entities in total)
• 機能のキーワード: multi copper oxidase, laccase, complex with molecular oxygen, oxidoreductase
• 由来する生物種: Steccherinum murashkinskyi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59964.95

構造登録者

Polyakov, K.M.,Gavryushov, S.,Fedorova, T.V.,Glazunova, O.A.,Popov, A.N. (登録日: 2016-11-27, 公開日: 2017-05-10, 最終更新日: 2024-01-17)

主引用文献

Polyakov, K.M.,Gavryushov, S.,Ivanova, S.,Fedorova, T.V.,Glazunova, O.A.,Popov, A.N.,Koroleva, O.V.
Structural study of the X-ray-induced enzymatic reduction of molecular oxygen to water by Steccherinum murashkinskyi laccase: insights into the reaction mechanism.
Acta Crystallogr D Struct Biol, 73:388-401, 2017

PubMed Abstract: The laccase from Steccherinum murashkinskyi is a member of the large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates, accompanied by the reduction of dioxygen to water. The reducing properties of X-ray radiation and the high quality of the laccase crystals allow the study of the catalytic reduction of dioxygen to water directly in a crystal. A series of diffraction data sets with increasing absorbed radiation dose were collected from a single crystal of Steccherinum murashkinskyi laccase at 1.35 Å resolution. Changes in the active-site structure associated with the reduction of molecular oxygen to water on increasing the absorbed dose of ionizing radiation were detected. The structures in the series are mixtures of different states of the enzyme-substrate complex. Nevertheless, it was possible to interpret these structures as complexes of various oxygen ligands with copper ions in different oxidation states. The results allowed the mechanism of oxygen reduction catalyzed by laccases to be refined.

PubMed: 28471364
DOI: 10.1107/S2059798317003667

実験手法

X-RAY DIFFRACTION (1.35 Å)