5MGA

Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage

Summary for 5MGA

DescriptorCRISPR-associated endonuclease Cpf1, RNA (40-MER), DNA (26-MER), ... (6 entities in total)
Functional Keywordsfncpf1, cpf1, r-loop, crispr, hydrolase
Biological sourceFrancisella tularensis subsp. novicida U112
Total number of polymer chains4
Total molecular weight179330.73
Authors
Montoya, G.,Stella, S. (deposition date: 2016-11-21, release date: 2017-06-21, Last modification date: 2018-10-24)
Primary citation
Stella, S.,Alcon, P.,Montoya, G.
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage.
Nature, 546:559-563, 2017
PubMed: 28562584 (PDB entries with the same primary citation)
DOI: 10.1038/nature22398
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.26118 1.2% 8.0% 2.2% 0.56MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution