5MC6

Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae

これはPDB形式変換不可エントリーです。

5MC6 の概要

• エントリーDOI: 10.2210/pdb5mc6/pdb
• EMDBエントリー: 3461
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein S19-A, 40S ribosomal protein S20, ... (86 entities in total)
• 機能のキーワード: cryo-em, ribosome, rna, helicase
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 87
• 化学式量合計: 3599383.47

構造登録者

Schmidt, C.,Kowalinski, E.,Shanmuganathan, V.,Defenouillere, Q.,Braunger, K.,Heuer, A.,Pech, M.,Namane, A.,Berninghausen, O.,Fromont-Racine, M.,Jacquier, A.,Conti, E.,Becker, T.,Beckmann, R. (登録日: 2016-11-09, 公開日: 2017-01-18, 最終更新日: 2025-07-09)

主引用文献

Schmidt, C.,Kowalinski, E.,Shanmuganathan, V.,Defenouillere, Q.,Braunger, K.,Heuer, A.,Pech, M.,Namane, A.,Berninghausen, O.,Fromont-Racine, M.,Jacquier, A.,Conti, E.,Becker, T.,Beckmann, R.
The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex.
Science, 354:1431-1433, 2016

PubMed Abstract: Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski complex directly associates with 80S ribosomes presenting a short mRNA 3' overhang. We determined the structure of an endogenous ribosome-Ski complex using cryo-electron microscopy (EM) with a local resolution of the Ski complex ranging from 4 angstroms (Å) in the core to about 10 Å for intrinsically flexible regions. Ribosome binding displaces the autoinhibitory domain of the Ski2 helicase, positioning it in an open conformation near the ribosomal mRNA entry tunnel. We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation.

PubMed: 27980209
DOI: 10.1126/science.aaf7520

実験手法

ELECTRON MICROSCOPY (3.8 Å)