5MBU

CeuE (H227A, Y288F variant) a periplasmic protein from Campylobacter jejuni

5MBU の概要

• エントリーDOI: 10.2210/pdb5mbu/pdb
• 分子名称: Enterochelin uptake periplasmic binding protein (3 entities in total)
• 機能のキーワード: periplasmic, iron-uptake, tetradentate, siderophore, mutation, metal transport
• 由来する生物種: Campylobacter jejuni; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 95996.76

構造登録者

Wilde, E.J.,Blagova, E.V.,Hughes, A.,Raines, D.J.,Moroz, O.V.,Turkenburg, J.P.,Duhme-Klair, A.-K.,Wilson, K.S. (登録日: 2016-11-08, 公開日: 2017-04-12, 最終更新日: 2024-01-17)

主引用文献

Wilde, E.J.,Hughes, A.,Blagova, E.V.,Moroz, O.V.,Thomas, R.P.,Turkenburg, J.P.,Raines, D.J.,Duhme-Klair, A.K.,Wilson, K.S.
Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM(4-) siderophore analogues of varied linker length.
Sci Rep, 7:45941-45941, 2017

PubMed Abstract: Bacteria use siderophores to mediate the transport of essential Fe(III) into the cell. In Campylobacter jejuni the periplasmic binding protein CeuE, an integral part of the Fe(III) transport system, has adapted to bind tetradentate siderophores using a His and a Tyr side chain to complete the Fe(III) coordination. A series of tetradentate siderophore mimics was synthesized in which the length of the linker between the two iron-binding catecholamide units was increased from four carbon atoms (4-LICAM) to five, six and eight (5-, 6-, 8-LICAM, respectively). Co-crystal structures with CeuE showed that the inter-planar angles between the iron-binding catecholamide units in the 5-, 6- and 8-LICAM structures are very similar (111°, 110° and 110°) and allow for an optimum fit into the binding pocket of CeuE, the inter-planar angle in the structure of 4-LICAM is significantly smaller (97°) due to restrictions imposed by the shorter linker. Accordingly, the protein-binding affinity was found to be slightly higher for 5- compared to 4-LICAM but decreases for 6- and 8-LICAM. The optimum linker length of five matches that present in natural siderophores such as enterobactin and azotochelin. Site-directed mutagenesis was used to investigate the relative importance of the Fe(III)-coordinating residues H227 and Y288.

PubMed: 28383577
DOI: 10.1038/srep45941

実験手法

X-RAY DIFFRACTION (1.81 Å)