5MB4
Crystal Structure of the Psathyrella asperospora lectin PAL in complex with GlcNAc
Summary for 5MB4
| Entry DOI | 10.2210/pdb5mb4/pdb |
| Descriptor | GlcNAc specific lectin, SODIUM ION, MALONATE ION, ... (6 entities in total) |
| Functional Keywords | lectin, fungi, seven bladed beta propeller, glcnac, sugar binding protein |
| Biological source | Psathyrella |
| Total number of polymer chains | 1 |
| Total formula weight | 44716.28 |
| Authors | Ribeiro, J.,Varrot, A. (deposition date: 2016-11-07, release date: 2017-02-22, Last modification date: 2024-01-17) |
| Primary citation | Ribeiro, J.P.,Ali Abol Hassan, M.,Rouf, R.,Tiralongo, E.,May, T.W.,Day, C.J.,Imberty, A.,Tiralongo, J.,Varrot, A. Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin. Proteins, 85:969-975, 2017 Cited by PubMed Abstract: A lectin with strong cytotoxic effect on human colon cancer HT29 and monkey kidney VERO cells was recently identified from the Australian indigenous mushroom Psathyrella asperospora and named PAL. We herein present its biochemical and structural analysis using a multidisciplinary approach. Glycan arrays revealed binding preference towards N-acetylglucosamine (GlcNAc) and, to a lesser extent, towards sialic acid (Neu5Ac). Submicromolar and millimolar affinity was measured by surface plasmon resonance for GlcNAc and NeuAc, respectively. The structure of PAL was resolved by X-ray crystallography, elucidating both the protein's amino acid sequence as well as the molecular basis rationalizing its binding specificity. Proteins 2017; 85:969-975. © 2016 Wiley Periodicals, Inc. PubMed: 28168856DOI: 10.1002/prot.25265 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
