5MB4

Crystal Structure of the Psathyrella asperospora lectin PAL in complex with GlcNAc

5MB4 の概要

• エントリーDOI: 10.2210/pdb5mb4/pdb
• 分子名称: GlcNAc specific lectin, SODIUM ION, MALONATE ION, ... (6 entities in total)
• 機能のキーワード: lectin, fungi, seven bladed beta propeller, glcnac, sugar binding protein
• 由来する生物種: Psathyrella
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44716.28

構造登録者

Ribeiro, J.,Varrot, A. (登録日: 2016-11-07, 公開日: 2017-02-22, 最終更新日: 2024-01-17)

主引用文献

Ribeiro, J.P.,Ali Abol Hassan, M.,Rouf, R.,Tiralongo, E.,May, T.W.,Day, C.J.,Imberty, A.,Tiralongo, J.,Varrot, A.
Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin.
Proteins, 85:969-975, 2017

PubMed Abstract: A lectin with strong cytotoxic effect on human colon cancer HT29 and monkey kidney VERO cells was recently identified from the Australian indigenous mushroom Psathyrella asperospora and named PAL. We herein present its biochemical and structural analysis using a multidisciplinary approach. Glycan arrays revealed binding preference towards N-acetylglucosamine (GlcNAc) and, to a lesser extent, towards sialic acid (Neu5Ac). Submicromolar and millimolar affinity was measured by surface plasmon resonance for GlcNAc and NeuAc, respectively. The structure of PAL was resolved by X-ray crystallography, elucidating both the protein's amino acid sequence as well as the molecular basis rationalizing its binding specificity. Proteins 2017; 85:969-975. © 2016 Wiley Periodicals, Inc.

PubMed: 28168856
DOI: 10.1002/prot.25265

実験手法

X-RAY DIFFRACTION (2 Å)