5MAN

Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to nigerose

5MAN の概要

• エントリーDOI: 10.2210/pdb5man/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900052
• 分子名称: Sucrose phosphorylase, alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: sucrose phosphorylase, resveratrol, enzyme design, transferase
• 由来する生物種: Bifidobacterium adolescentis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56613.02

構造登録者

Grimm, C.,Kraus, M. (登録日: 2016-11-03, 公開日: 2017-12-20, 最終更新日: 2024-05-08)

主引用文献

Kraus, M.,Grimm, C.,Seibel, J.
Switching enzyme specificity from phosphate to resveratrol glucosylation.
Chem. Commun. (Camb.), 53:12181-12184, 2017

PubMed Abstract: Here we present a point mutation-triggered domain shift which switches the acceptor preference of a sucrose phosphorylase from phosphate to a variety of large polyphenolic compounds including resveratrol and quercetin, enabling their efficient glucosylation. The variant possesses a high affinity for aromatic substrates due to newly introduced π-π- and hydrophobic interactions in the altered active site. The domain shift brings about a substantially enlarged and multifunctional active site for polyphenol glucosylation and rare disaccharide production. The crystal structure of the variant with its product resveratrol-3-α-d-glucoside allows the prediction of the substrate scope and regioselectivity of the aromatic compounds' glucosylation sites.

PubMed: 29057405
DOI: 10.1039/c7cc05993k

実験手法

X-RAY DIFFRACTION (2.04 Å)