5M9Z

Second zinc-binding domain from yeast Pcf11

5M9Z の概要

• エントリーDOI: 10.2210/pdb5m9z/pdb
• NMR情報: BMRB: 34061
• 分子名称: Protein PCF11, ZINC ION (2 entities in total)
• 機能のキーワード: zinc-binding, mrna, rna processing, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10904.40

構造登録者

Mackereth, C. (登録日: 2016-11-02, 公開日: 2017-08-02, 最終更新日: 2024-05-15)

主引用文献

Guegueniat, J.,Dupin, A.F.,Stojko, J.,Beaurepaire, L.,Cianferani, S.,Mackereth, C.D.,Minvielle-Sebastia, L.,Fribourg, S.
Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing.
Nucleic Acids Res., 45:10115-10131, 2017

PubMed Abstract: New transcripts generated by RNA polymerase II (RNAPII) are generally processed in order to form mature mRNAs. Two key processing steps include a precise cleavage within the 3' end of the pre-mRNA, and the subsequent polymerization of adenosines to produce the poly(A) tail. In yeast, these two functions are performed by a large multi-subunit complex that includes the Cleavage Factor IA (CF IA). The four proteins Pcf11, Clp1, Rna14 and Rna15 constitute the yeast CF IA, and of these, Pcf11 is structurally the least characterized. Here, we provide evidence for the binding of two Zn2+ atoms to Pcf11, bound to separate zinc-binding domains located on each side of the Clp1 recognition region. Additional structural characterization of the second zinc-binding domain shows that it forms an unusual zinc finger fold. We further demonstrate that the two domains are not mandatory for CF IA assembly nor RNA polymerase II transcription termination, but are rather involved to different extents in the pre-mRNA 3'-end processing mechanism. Our data thus contribute to a more complete understanding of the architecture and function of Pcf11 and its role within the yeast CF IA complex.

PubMed: 28973460
DOI: 10.1093/nar/gkx674

実験手法

SOLUTION NMR