5M8T

Crystal structure of human tyrosinase related protein 1 (T391V-R374S-Y362F) in complex with tropolone

5M8T の概要

• エントリーDOI: 10.2210/pdb5m8t/pdb
• 分子名称: 5,6-dihydroxyindole-2-carboxylic acid oxidase, 2-HYDROXYCYCLOHEPTA-2,4,6-TRIEN-1-ONE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: human tyrosinase related protein 1, melanin biosynthesis, tyrosinase, oxidoreductase, unknown function
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 213378.02

構造登録者

Lai, X.,Soler-Lopez, M.,Wichers, H.J.,Dijkstra, B.W. (登録日: 2016-10-29, 公開日: 2017-07-12, 最終更新日: 2024-10-23)

主引用文献

Lai, X.,Wichers, H.J.,Soler-Lopez, M.,Dijkstra, B.W.
Structure of Human Tyrosinase Related Protein 1 Reveals a Binuclear Zinc Active Site Important for Melanogenesis.
Angew. Chem. Int. Ed. Engl., 56:9812-9815, 2017

PubMed Abstract: Tyrosinase-related protein 1 (TYRP1) is one of three tyrosinase-like glycoenzymes in human melanocytes that are key to the production of melanin, the compound responsible for the pigmentation of skin, eye, and hair. Difficulties with producing these enzymes in pure form have hampered the understanding of their activity and the effect of mutations that cause albinism and pigmentation disorders. Herein we show that the typical tyrosinase-like subdomain of TYRP1 contains two zinc ions in the active site instead of copper ions as found in tyrosinases, which explains why TYRP1 does not exhibit tyrosinase redox activity. In addition, the structures reveal for the first time that the Cys-rich subdomain, which is unique to vertebrate melanogenic proteins, has an epidermal growth factor-like fold and is tightly associated with the tyrosinase subdomain. Our structures suggest that most albinism-related mutations of TYRP1 affect its stability or activity.

PubMed: 28661582
DOI: 10.1002/anie.201704616

実験手法

X-RAY DIFFRACTION (2.35 Å)