5M5H

RIBOSOME-BOUND YIDC INSERTASE

5M5H の概要

• エントリーDOI: 10.2210/pdb5m5h/pdb
• EMDBエントリー: 4155
• 分子名称: Membrane protein insertase YidC (1 entity in total)
• 機能のキーワード: membrane protein, nanodisc, ribosome ligand, protein transport
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61872.70

構造登録者

Kedrov, A.,Wickles, S.,Crevenna, A.H.,van der Sluis, E.,Buschauer, R.,Berninghausen, O.,Lamb, D.C.,Beckmann, R. (登録日: 2016-10-21, 公開日: 2016-12-14, 最終更新日: 2024-05-08)

主引用文献

Kedrov, A.,Wickles, S.,Crevenna, A.H.,van der Sluis, E.O.,Buschauer, R.,Berninghausen, O.,Lamb, D.C.,Beckmann, R.
Structural Dynamics of the YidC:Ribosome Complex during Membrane Protein Biogenesis.
Cell Rep, 17:2943-2954, 2016

PubMed Abstract: Members of the YidC/Oxa1/Alb3 family universally facilitate membrane protein biogenesis, via mechanisms that have thus far remained unclear. Here, we investigated two crucial functional aspects: the interaction of YidC with ribosome:nascent chain complexes (RNCs) and the structural dynamics of RNC-bound YidC in nanodiscs. We observed that a fully exposed nascent transmembrane domain (TMD) is required for high-affinity YidC:RNC interactions, while weaker binding may already occur at earlier stages of translation. YidC efficiently catalyzed the membrane insertion of nascent TMDs in both fluid and gel phase membranes. Cryo-electron microscopy and fluorescence analysis revealed a conformational change in YidC upon nascent chain insertion: the essential TMDs 2 and 3 of YidC were tilted, while the amphipathic helix EH1 relocated into the hydrophobic core of the membrane. We suggest that EH1 serves as a mechanical lever, facilitating a coordinated movement of YidC TMDs to trigger the release of nascent chains into the membrane.

PubMed: 27974208
DOI: 10.1016/j.celrep.2016.11.059

実験手法

ELECTRON MICROSCOPY (4.5 Å)