5M50

Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins

5M50 の概要

• エントリーDOI: 10.2210/pdb5m50/pdb
• EMDBエントリー: 4154
• 分子名称: Tubulin alpha chain, Tubulin beta-2B chain, Calmodulin-regulated spectrin-associated protein 3, ... (8 entities in total)
• 機能のキーワード: camsap ckk microtubule tubulin, structural protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton : F2Z4C1 Q6B856; Cell junction, adherens junction : Q80VC9
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 210542.44

構造登録者

Akhmanova, A.,Moores, C.A.,Baldus, M.,Steinmetz, M.O.,Topf, M.,Roberts, A.J.,Grant, B.J.,Scarabelli, G.,Joseph, A.-P.,van Hooff, J.J.E.,Houben, K.,Hua, S.,Luo, Y.,Stangier, M.M.,Jiang, K.,Atherton, J. (登録日: 2016-10-20, 公開日: 2017-10-04, 最終更新日: 2024-05-15)

主引用文献

Atherton, J.,Jiang, K.,Stangier, M.M.,Luo, Y.,Hua, S.,Houben, K.,van Hooff, J.J.E.,Joseph, A.P.,Scarabelli, G.,Grant, B.J.,Roberts, A.J.,Topf, M.,Steinmetz, M.O.,Baldus, M.,Moores, C.A.,Akhmanova, A.
A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
Nat. Struct. Mol. Biol., 24:931-943, 2017

PubMed Abstract: CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we found that the CAMSAP C-terminal CKK domain is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncovered how mammalian CKK binds between two tubulin dimers at the interprotofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high-resolution fluorescence microscopy and cryo-electron tomography suggested that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the interprotofilament interface at this site serve as the basis for CKK's minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends.

PubMed: 28991265
DOI: 10.1038/nsmb.3483

実験手法

ELECTRON MICROSCOPY (5.3 Å)