5M41

Crystal structure of nigritoxine

5M41 の概要

• エントリーDOI: 10.2210/pdb5m41/pdb
• 分子名称: Nigritoxine, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: toxin, new fold, arthropod
• 由来する生物種: Vibrio nigripulchritudo
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 83025.70

構造登録者

Czjzek, M.,Labreuche, L.,Jeudy, A.,Le Roux, F. (登録日: 2016-10-17, 公開日: 2017-12-06, 最終更新日: 2024-05-08)

主引用文献

Labreuche, Y.,Chenivesse, S.,Jeudy, A.,Le Panse, S.,Boulo, V.,Ansquer, D.,Pages, S.,Givaudan, A.,Czjzek, M.,Le Roux, F.
Nigritoxin is a bacterial toxin for crustaceans and insects.
Nat Commun, 8:1248-1248, 2017

PubMed Abstract: The Tetraconata (Pancrustacea) concept proposes that insects are more closely related to aquatic crustaceans than to terrestrial centipedes or millipedes. The question therefore arises whether insects have kept crustacean-specific genetic traits that could be targeted by specific toxins. Here we show that a toxin (nigritoxin), originally identified in a bacterial pathogen of shrimp, is lethal for organisms within the Tetraconata and non-toxic to other animals. X-ray crystallography reveals that nigritoxin possesses a new protein fold of the α/β type. The nigritoxin N-terminal domain is essential for cellular translocation and likely encodes specificity for Tetraconata. Once internalized by eukaryotic cells, nigritoxin induces apoptotic cell death through structural features that are localized in the C-terminal domain of the protein. We propose that nigritoxin will be an effective means to identify a Tetraconata evolutionarily conserved pathway and speculate that nigritoxin holds promise as an insecticidal protein.

PubMed: 29093459
DOI: 10.1038/s41467-017-01445-z

実験手法

X-RAY DIFFRACTION (2.1 Å)