5M3Q
Crystal structure of Tif6 from Chaetomium thermophilum
Summary for 5M3Q
Entry DOI | 10.2210/pdb5m3q/pdb |
Related | 5M43 |
Descriptor | Eukaryotic translation initiation factor 6, SULFATE ION, GLYCEROL, ... (4 entities in total) |
Functional Keywords | ribosome biogenesis, anti-association factor, ribosome |
Biological source | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) |
Cellular location | Cytoplasm : G0S683 |
Total number of polymer chains | 1 |
Total formula weight | 27914.23 |
Authors | Ahmed, Y.L.,Calvino, F.R.,Sinning, I. (deposition date: 2016-10-17, release date: 2016-11-30, Last modification date: 2024-01-17) |
Primary citation | Baler, J.,Ahmed, Y.L.,Kallas, M.,Kornprobst, M.,Calvino, F.R.,Gnadig, M.,Thoms, M.,Stier, G.,Ismail, S.,Kharde, S.,Castillo, N.,Griesel, S.,Bastuck, S.,Bradatsch, B.,Thomson, E.,Flemming, D.,Sinning, I.,Hurt, E. Interaction network of the ribosome assembly machinery from a eukaryotic thermophile. Protein Sci., 26:327-342, 2017 Cited by PubMed Abstract: Ribosome biogenesis in eukaryotic cells is a highly dynamic and complex process innately linked to cell proliferation. The assembly of ribosomes is driven by a myriad of biogenesis factors that shape pre-ribosomal particles by processing and folding the ribosomal RNA and incorporating ribosomal proteins. Biochemical approaches allowed the isolation and characterization of pre-ribosomal particles from Saccharomyces cerevisiae, which lead to a spatiotemporal map of biogenesis intermediates along the path from the nucleolus to the cytoplasm. Here, we cloned almost the entire set (∼180) of ribosome biogenesis factors from the thermophilic fungus Chaetomium thermophilum in order to perform an in-depth analysis of their protein-protein interaction network as well as exploring the suitability of these thermostable proteins for structural studies. First, we performed a systematic screen, testing about 80 factors for crystallization and structure determination. Next, we performed a yeast 2-hybrid analysis and tested about 32,000 binary combinations, which identified more than 1000 protein-protein contacts between the thermophilic ribosome assembly factors. To exemplary verify several of these interactions, we performed biochemical reconstitution with the focus on the interaction network between 90S pre-ribosome factors forming the ctUTP-A and ctUTP-B modules, and the Brix-domain containing assembly factors of the pre-60S subunit. Our work provides a rich resource for biochemical reconstitution and structural analyses of the conserved ribosome assembly machinery from a eukaryotic thermophile. PubMed: 27863450DOI: 10.1002/pro.3085 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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