5M33

Structural tuning of CD81LEL (space group P21)

Summary for 5M33

• Entry DOI: 10.2210/pdb5m33/pdb
• Related: 1G8Q 1IV5 5M2C 5M3D 5M3T 5M4R
• Descriptor: CD81 antigen, 1,2-ETHANEDIOL (3 entities in total)
• Functional Keywords: human cellular receptor for hepatitis c virus, signaling protein, cell adhesion
• Biological source: Homo sapiens (Human)
• Cellular location: Basolateral cell membrane ; Multi-pass membrane protein : P60033
• Total number of polymer chains: 2
• Total formula weight: 22799.31

Authors

Cunha, E.S.,Sfriso, P.,Rojas, A.L.,Roversi, P.,Hospital, A.,Orozco, M.,Abrescia, N.G. (deposition date: 2016-10-14, release date: 2016-12-14, Last modification date: 2024-10-16)

Primary citation

Cunha, E.S.,Sfriso, P.,Rojas, A.L.,Roversi, P.,Hospital, A.,Orozco, M.,Abrescia, N.G.
Mechanism of Structural Tuning of the Hepatitis C Virus Human Cellular Receptor CD81 Large Extracellular Loop.
Structure, 25:53-65, 2017

PubMed Abstract: Hepatitis C virus (HCV) enters into human hepatocytes via tetraspanin hCD81. HCV glycoprotein E2 recognizes the "head" subdomain of the large extracellular loop (LEL) of CD81 (hCD81), but the precise mechanism of virus cell attachment and entry remains elusive. Here, by combining the structural analysis of a conspicuous number of crystallized CD81 molecules with molecular dynamics simulations, we show that the conformational plasticity of the hCD81 head subdomain is a molecular property of the receptor. The observed closed, intermediate, and open conformations of the head subdomain provide distinct binding platforms. Simulations at pH 7.4 and 4.0 indicate that this dynamism is pH modulated. The crystallized double conformation of the disulfide bridge C157-C175 at the base of the head subdomain identifies this bond as the molecular zipper of the plasticity of hCD81. We propose that this conformational dependence of hCD81, which is finely tuned by pH and redox conditions, enables the virus-receptor interactions to diversely re-engage at endosomal conditions.

PubMed: 27916518
DOI: 10.1016/j.str.2016.11.003

Experimental method

X-RAY DIFFRACTION (1.28 Å)