5M2W

Structure of nanobody nb18 raised against TssK from E. coli T6SS

5M2W の概要

• エントリーDOI: 10.2210/pdb5m2w/pdb
• 分子名称: Llama nanobody nb8 against TssK from T6SS, SULFATE ION (3 entities in total)
• 機能のキーワード: t6ss tssk nanobody, immune system
• 由来する生物種: Lama glama (Llama)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27698.76

構造登録者

Cambillau, C.,Nguyen, V.S.,Spinelli, S.,Desmyter, A. (登録日: 2016-10-13, 公開日: 2017-06-28, 最終更新日: 2024-11-06)

主引用文献

Nguyen, V.S.,Logger, L.,Spinelli, S.,Legrand, P.,Huyen Pham, T.T.,Nhung Trinh, T.T.,Cherrak, Y.,Zoued, A.,Desmyter, A.,Durand, E.,Roussel, A.,Kellenberger, C.,Cascales, E.,Cambillau, C.
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex.
Nat Microbiol, 2:17103-17103, 2017

PubMed Abstract: The type VI secretion system (T6SS) is a multiprotein machine widespread in Gram-negative bacteria that delivers toxins into both eukaryotic and prokaryotic cells. The mechanism of action of the T6SS is comparable to that of contractile myophages. The T6SS builds a tail-like structure made of an inner tube wrapped by a sheath, assembled under an extended conformation. Contraction of the sheath propels the inner tube towards the target cell. The T6SS tail is assembled on a platform-the baseplate-which is functionally similar to bacteriophage baseplates. In addition, the baseplate docks the tail to a trans-envelope membrane complex that orients the tail towards the target. Here, we report the crystal structure of TssK, a central component of the T6SS baseplate. We show that TssK is composed of three domains, and establish the contribution of each domain to the interaction with TssK partners. Importantly, this study reveals that the N-terminal domain of TssK is structurally homologous to the shoulder domain of phage receptor-binding proteins, and the C-terminal domain binds the membrane complex. We propose that TssK has conserved the domain of attachment to the virion particle but has evolved the reception domain to use the T6SS membrane complex as receptor.

PubMed: 28650463
DOI: 10.1038/nmicrobiol.2017.103

実験手法

X-RAY DIFFRACTION (1.5 Å)