5LY3

P. calidifontis crenactin in complex with arcadin-2 C-terminal peptide

5LY3 の概要

• エントリーDOI: 10.2210/pdb5ly3/pdb
• 分子名称: Actin/actin family protein, Actin-like protein, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: actin, bacterial cytoskeleton, structural protein
• 由来する生物種: Pyrobaculum calidifontis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50698.82

構造登録者

Izore, T.,Lowe, J. (登録日: 2016-09-23, 公開日: 2016-11-30, 最終更新日: 2024-01-17)

主引用文献

Izore, T.,Kureisaite-Ciziene, D.,McLaughlin, S.H.,Lowe, J.
Crenactin forms actin-like double helical filaments regulated by arcadin-2.
Elife, 5:-, 2016

PubMed Abstract: The similarity of eukaryotic actin to crenactin, a filament-forming protein from the crenarchaeon supports the theory of a common origin of Crenarchaea and Eukaryotes. Monomeric structures of crenactin and actin are similar, although their filament architectures were suggested to be different. Here we report that crenactin forms double helical filaments that show exceptional similarity to eukaryotic F-actin. With cryo-electron microscopy and helical reconstruction we solved the structure of the crenactin filament to 3.8 Å resolution. When forming double filaments, the 'hydrophobic plug' loop in crenactin rearranges. Arcadin-2, also encoded by the arcade gene cluster, binds tightly with its C-terminus to the hydrophobic groove of crenactin. Binding is reminiscent of eukaryotic actin modulators such as cofilin and thymosin β4 and arcadin-2 is a depolymeriser of crenactin filaments. Our work further supports the theory of shared ancestry of Eukaryotes and Crenarchaea.

PubMed: 27852434
DOI: 10.7554/eLife.21600

実験手法

X-RAY DIFFRACTION (1.6 Å)