5LXK

NMR structure of the C-terminal domain of the Bacteriophage T5 decoration protein pb10.

5LXK の概要

• エントリーDOI: 10.2210/pdb5lxk/pdb
• NMR情報: BMRB: 34046
• 分子名称: Decoration protein (1 entity in total)
• 機能のキーワード: bacteriophage t5 decoration protein, viral protein
• 由来する生物種: Escherichia phage T5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10339.29

構造登録者

Vernhes, E.,Gilquin, B.,Cuniasse, P.,Boulanger, P.,Zinn-Justin, S. (登録日: 2016-09-22, 公開日: 2017-08-02, 最終更新日: 2024-05-15)

主引用文献

Vernhes, E.,Renouard, M.,Gilquin, B.,Cuniasse, P.,Durand, D.,England, P.,Hoos, S.,Huet, A.,Conway, J.F.,Glukhov, A.,Ksenzenko, V.,Jacquet, E.,Nhiri, N.,Zinn-Justin, S.,Boulanger, P.
High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid.
Sci Rep, 7:41662-41662, 2017

PubMed Abstract: Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major capsid protein. Here we determined the 3D structure of pb10 and investigated its capsid-binding properties using NMR, SAXS, cryoEM and SPR. Pb10 consists of an α-helical capsid-binding domain and an Ig-like domain exposed to the solvent. It binds to the T5 capsid with a remarkably high affinity and its binding kinetics is characterized by a very slow dissociation rate. We propose that the conformational exchange events observed in the capsid-binding domain enable rearrangements upon binding that contribute to the quasi-irreversibility of the pb10-capsid interaction. Moreover we show that pb10 binding is a highly cooperative process, which favours immediate rebinding of newly dissociated pb10 to the 120 hexamers of the capsid protein. In extreme conditions, pb10 protects the phage from releasing its genome. We conclude that pb10 may function to reinforce the capsid thus favouring phage survival in harsh environments.

PubMed: 28165000
DOI: 10.1038/srep41662

実験手法

SOLUTION NMR