5LW8

NMR solution structure of Helicobacter pylori TonB-CTD (residues 194-285)

5LW8 の概要

• エントリーDOI: 10.2210/pdb5lw8/pdb
• NMR情報: BMRB: 34043
• 分子名称: Protein TonB (1 entity in total)
• 機能のキーワード: tonb-dependent iron uptake, c-terminal domain, proline rich domain, metal transport
• 由来する生物種: Helicobacter pylori (strain ATCC 700392 / 26695)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10151.67

構造登録者

Ciragan, A.,Aranko, A.S.,Tascon, I.,Iwai, H. (登録日: 2016-09-15, 公開日: 2016-10-19, 最終更新日: 2024-06-19)

主引用文献

Ciragan, A.,Aranko, A.S.,Tascon, I.,Iwai, H.
Salt-inducible Protein Splicing in cis and trans by Inteins from Extremely Halophilic Archaea as a Novel Protein-Engineering Tool.
J.Mol.Biol., 428:4573-4588, 2016

PubMed Abstract: Intervening protein sequences (inteins) from extremely halophilic haloarchaea can be inactive under low salinity but could be activated by increasing the salt content to a specific concentration for each intein. The halo-obligatory inteins confer high solubility under both low and high salinity conditions. We showed the broad utility of salt-dependent protein splicing in cis and trans by demonstrating backbone cyclization, self-cleavage for purification, and scarless protein ligation for segmental isotopic labeling. Artificially split MCM2 intein derived from Halorhabdus utahensis remained highly soluble and was capable of protein trans-splicing with excellent ligation kinetics by reassembly under high salinity conditions. Importantly, the MCM2 intein has the active site residue of Ser at the +1 position, which remains in the ligated product, instead of Cys as found in many other efficient split inteins. Since Ser is more abundant than Cys in proteins, the novel split intein could widen the applications of segmental labeling in protein NMR spectroscopy and traceless protein ligation by exploiting a Ser residue in the native sequences as the +1 position of the MCM2 intein. The split halo-obligatory intein was successfully used to demonstrate the utility in NMR investigation of intact proteins by producing segmentally isotope-labeled intact TonB protein from Helicobacter pylori.

PubMed: 27720988
DOI: 10.1016/j.jmb.2016.10.006

実験手法

SOLUTION NMR