5LVW

XiaF (FADH2) from Streptomyces sp.

5LVW の概要

• エントリーDOI: 10.2210/pdb5lvw/pdb
• 関連するPDBエントリー: 2JBR
• 分子名称: XiaF protein, FLAVIN-ADENINE DINUCLEOTIDE, INDOLE, ... (5 entities in total)
• 機能のキーワード: detoxification, non-canonical flavin-dependent terpenoid cyclase, xenobiotics-degrading enzyme, indigo, indirubin, oxidoreductase
• 由来する生物種: Streptomyces sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45238.51

構造登録者

Kugel, S.,Baunach, M.,Baer, P.,Ishida-Ito, M.,Sundaram, S.,Xu, Z.,Groll, M.,Hertweck, C. (登録日: 2016-09-14, 公開日: 2017-06-28, 最終更新日: 2024-01-17)

主引用文献

Kugel, S.,Baunach, M.,Baer, P.,Ishida-Ito, M.,Sundaram, S.,Xu, Z.,Groll, M.,Hertweck, C.
Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification.
Nat Commun, 8:15804-15804, 2017

PubMed Abstract: Terpenoid natural products comprise a wide range of molecular architectures that typically result from C-C bond formations catalysed by classical type I/II terpene cyclases. However, the molecular diversity of biologically active terpenoids is substantially increased by fully unrelated, non-canonical terpenoid cyclases. Their evolutionary origin has remained enigmatic. Here we report the in vitro reconstitution of an unusual flavin-dependent bacterial indoloterpenoid cyclase, XiaF, together with a designated flavoenzyme-reductase (XiaP) that mediates a key step in xiamycin biosynthesis. The crystal structure of XiaF with bound FADH (at 2.4 Å resolution) and phylogenetic analyses reveal that XiaF is, surprisingly, most closely related to xenobiotic-degrading enzymes. Biotransformation assays show that XiaF is a designated indole hydroxylase that can be used for the production of indigo and indirubin. We unveil a cryptic hydroxylation step that sets the basis for terpenoid cyclization and suggest that the cyclase has evolved from xenobiotics detoxification enzymes.

PubMed: 28643772
DOI: 10.1038/ncomms15804

実験手法

X-RAY DIFFRACTION (2.9 Å)