5LSH

human lysozyme in complex with a tetrasaccharide fragment of the O-chain of LPS from Klebsiella pneumoniae

5LSH の概要

• エントリーDOI: 10.2210/pdb5lsh/pdb
• 分子名称: Lysozyme C, alpha-D-galactopyranose-(1-3)-beta-D-galactofuranose-(1-3)-alpha-D-galactopyranose-(1-3)-propyl beta-D-galactofuranoside, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: lectin, complex, lps, o-chain, sugar binding protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Secreted: P61626
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15558.70

構造登録者

Zhang, R.,Nifantiev, N.E.,Krylov, V.,Luetteke, T.,Scheidig, A.J.,Siebert, H.-C. (登録日: 2016-08-26, 公開日: 2017-06-21, 最終更新日: 2024-11-20)

主引用文献

Zhang, R.,Wu, L.,Eckert, T.,Burg-Roderfeld, M.,Rojas-Macias, M.A.,Lutteke, T.,Krylov, V.B.,Argunov, D.A.,Datta, A.,Markart, P.,Guenther, A.,Norden, B.,Schauer, R.,Bhunia, A.,Enani, M.A.,Billeter, M.,Scheidig, A.J.,Nifantiev, N.E.,Siebert, H.C.
Lysozyme's lectin-like characteristics facilitates its immune defense function.
Q. Rev. Biophys., 50:e9-e9, 2017

PubMed Abstract: Interactions between human lysozyme (HL) and the lipopolysaccharide (LPS) of Klebsiella pneumoniae O1, a causative agent of lung infection, were identified by surface plasmon resonance. To characterize the molecular mechanism of this interaction, HL binding to synthetic disaccharides and tetrasaccharides representing one and two repeating units, respectively, of the O-chain of this LPS were studied. pH-dependent structural rearrangements of HL after interaction with the disaccharide were observed through nuclear magnetic resonance. The crystal structure of the HL-tetrasaccharide complex revealed carbohydrate chain packing into the A, B, C, and D binding sites of HL, which primarily occurred through residue-specific, direct or water-mediated hydrogen bonds and hydrophobic contacts. Overall, these results support a crucial role of the Glu35/Asp53/Trp63/Asp102 residues in HL binding to the tetrasaccharide. These observations suggest an unknown glycan-guided mechanism that underlies recognition of the bacterial cell wall by lysozyme and may complement the HL immune defense function.

PubMed: 29233221
DOI: 10.1017/S0033583517000075

実験手法

X-RAY DIFFRACTION (1.061 Å)