5LS2

Receptor mediated chitin perception in legumes is functionally seperable from Nod factor perception

Summary for 5LS2

• Entry DOI: 10.2210/pdb5ls2/pdb
• Descriptor: LysM type receptor kinase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (4 entities in total)
• Functional Keywords: lysm domain, chitin binding, plant defence, plant protein
• Biological source: Lotus japonicus
• Total number of polymer chains: 2
• Total formula weight: 47323.82

Authors

Bozsoki, Z.,Cheng, J.,Feng, F.,Gysel, K.,Andersen, K.R.,Oldroyd, G.,Blaise, M.,Radutoiu, S.,Stougaard, J. (deposition date: 2016-08-22, release date: 2017-08-23, Last modification date: 2024-11-06)

Primary citation

Bozsoki, Z.,Cheng, J.,Feng, F.,Gysel, K.,Vinther, M.,Andersen, K.R.,Oldroyd, G.,Blaise, M.,Radutoiu, S.,Stougaard, J.
Receptor-mediated chitin perception in legume roots is functionally separable from Nod factor perception.
Proc. Natl. Acad. Sci. U.S.A., 114:E8118-E8127, 2017

PubMed Abstract: The ability of root cells to distinguish mutualistic microbes from pathogens is crucial for plants that allow symbiotic microorganisms to infect and colonize their internal root tissues. Here we show that and possess very similar LysM pattern-recognition receptors, LYS6/LYK9 and LYR4, enabling root cells to separate the perception of chitin oligomeric microbe-associated molecular patterns from the perception of lipochitin oligosaccharide by the NFR1/LYK3 and NFR5/NFP receptors triggering symbiosis. Inactivation of chitin-receptor genes in , , and mutants eliminates early reactive oxygen species responses and induction of defense-response genes in roots. , , and mutants were also more susceptible to fungal and bacterial pathogens, while infection and colonization by rhizobia and arbuscular mycorrhizal fungi was maintained. Biochemical binding studies with purified LYS6 ectodomains further showed that at least six GlcNAc moieties (CO6) are required for optimal binding efficiency. The 2.3-Å crystal structure of the LYS6 ectodomain reveals three LysM motifs similar to other LysM proteins and a conserved chitin-binding site. These results show that distinct receptor sets in legume roots respond to chitin and lipochitin oligosaccharides found in the heterogeneous mixture of chitinaceous compounds originating from soil microbes. This establishes a foundation for genetic and biochemical dissection of the perception and the downstream responses separating defense from symbiosis in the roots of the 80-90% of land plants able to develop rhizobial and/or mycorrhizal endosymbiosis.

PubMed: 28874587
DOI: 10.1073/pnas.1706795114

Experimental method

X-RAY DIFFRACTION (2.3 Å)