5LQT

RNA duplex has central consecutive GA pairs flanked by G-C basepairs

Summary for 5LQT

• Entry DOI: 10.2210/pdb5lqt/pdb
• Descriptor: RNA (5'-R(*GP*(CBV)P*CP*GP*GP*(6MZ)P*CP*GP*GP*C)-3'), CALCIUM ION, SODIUM ION, ... (5 entities in total)
• Functional Keywords: n6-methyladenine, rna folding, watson-crick basepairs, rna
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 6759.53

Authors

Huang, L.,Lilley, D.M.J. (deposition date: 2016-08-17, release date: 2017-06-21, Last modification date: 2024-05-08)

Primary citation

Huang, L.,Ashraf, S.,Wang, J.,Lilley, D.M.
Control of box C/D snoRNP assembly by N(6)-methylation of adenine.
EMBO Rep., 18:1631-1645, 2017

PubMed Abstract: N-methyladenine is the most widespread mRNA modification. A subset of human box C/D snoRNA species have target GAC sequences that lead to formation of N-methyladenine at a key Hoogsteen-sugar A·G base pair, of which half are methylated The GAC target is conserved only in those that are methylated. Methylation prevents binding of the 15.5-kDa protein and the induced folding of the RNA Thus, the assembly of the box C/D snoRNP could in principle be regulated by RNA methylation at its critical first stage. Crystallography reveals that N-methylation of adenine prevents the formation of Hoogsteen-sugar A·G base pairs, explaining why the box C/D RNA cannot adopt its kinked conformation. More generally, our data indicate that sheared A·G base pairs (but not Watson-Crick base pairs) are more susceptible to disruption by NmA methylation and are therefore possible regulatory sites. The human signal recognition particle RNA and many related Alu retrotransposon RNA species are also methylated at N6 of an adenine that forms a sheared base pair with guanine and mediates a key tertiary interaction.

PubMed: 28623187
DOI: 10.15252/embr.201743967

Experimental method

X-RAY DIFFRACTION (1.5 Å)