5LO7

Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica

5LO7 の概要

• エントリーDOI: 10.2210/pdb5lo7/pdb
• 分子名称: Fimbrial protein MyfA,Fimbrial protein MyfA, 2-(2-METHOXYETHOXY)ETHANOL (3 entities in total)
• 機能のキーワード: ig-like fold, beta sandwich, donor-strand complementation, cell adhesion
• 由来する生物種: Yersinia enterocolitica; 詳細
• 細胞内の位置: Fimbrium: P33406
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29231.54

構造登録者

Pakharukova, N.A.,Roy, S.,Tuitilla, M.,Zavialov, A.V. (登録日: 2016-08-08, 公開日: 2016-08-24, 最終更新日: 2024-01-10)

主引用文献

Pakharukova, N.,Roy, S.,Tuittila, M.,Rahman, M.M.,Paavilainen, S.,Ingars, A.K.,Skaldin, M.,Lamminmaki, U.,Hard, T.,Teneberg, S.,Zavialov, A.V.
Structural basis for Myf and Psa fimbriae-mediated tropism of pathogenic strains of Yersinia for host tissues.
Mol.Microbiol., 102:593-610, 2016

PubMed Abstract: Three pathogenic species of the genus Yersinia assemble adhesive fimbriae via the FGL-chaperone/usher pathway. Closely related Y. pestis and Y. pseudotuberculosis elaborate the pH6 antigen (Psa), which mediates bacterial attachment to alveolar cells of the lung. Y. enterocolitica, instead, assembles the homologous fimbriae Myf of unknown function. Here, we discovered that Myf, like Psa, specifically recognizes β1-3- or β1-4-linked galactose in glycosphingolipids, but completely lacks affinity for phosphatidylcholine, the main receptor for Psa in alveolar cells. The crystal structure of a subunit of Psa (PsaA) complexed with choline together with mutagenesis experiments revealed that PsaA has four phosphatidylcholine binding pockets that enable super-high-avidity binding of Psa-fibres to cell membranes. The pockets are arranged as six tyrosine residues, which are all missing in the MyfA subunit of Myf. Conversely, the crystal structure of the MyfA-galactose complex revealed that the galactose-binding site is more extended in MyfA, enabling tighter binding to lactosyl moieties. Our results suggest that during evolution, Psa has acquired a tyrosine-rich surface that enables it to bind to phosphatidylcholine and mediate adhesion of Y. pestis/pseudotuberculosis to alveolar cells, whereas Myf has specialized as a carbohydrate-binding adhesin, facilitating the attachment of Y. enterocolitica to intestinal cells.

PubMed: 27507539
DOI: 10.1111/mmi.13481

実験手法

X-RAY DIFFRACTION (1.9 Å)