5LN5

Crystal structure of the Wss1 E203Q mutant from S. pombe

Summary for 5LN5

• Entry DOI: 10.2210/pdb5ln5/pdb
• Descriptor: Ubiquitin and WLM domain-containing metalloprotease SPCC1442.07c, NICKEL (II) ION, CARBONATE ION, ... (4 entities in total)
• Functional Keywords: metalloprotease, dna-repair, endoprotease, regulation, mutant, hydrolase
• Biological source: Schizosaccharomyces pombe (Fission yeast)
• Cellular location: Cytoplasm : O94580
• Total number of polymer chains: 2
• Total formula weight: 30393.26

Authors

Groll, M.,Stingele, J.,Boulton, S.J. (deposition date: 2016-08-03, release date: 2016-11-09, Last modification date: 2024-01-10)

Primary citation

Stingele, J.,Bellelli, R.,Alte, F.,Hewitt, G.,Sarek, G.,Maslen, S.L.,Tsutakawa, S.E.,Borg, A.,Kjr, S.,Tainer, J.A.,Skehel, J.M.,Groll, M.,Boulton, S.J.
Mechanism and Regulation of DNA-Protein Crosslink Repair by the DNA-Dependent Metalloprotease SPRTN.
Mol.Cell, 64:688-703, 2016

PubMed Abstract: Covalent DNA-protein crosslinks (DPCs) are toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription. Little was known about DPC-specific repair mechanisms until the recent identification of a DPC-processing protease in yeast. The existence of a DPC protease in higher eukaryotes is inferred from data in Xenopus laevis egg extracts, but its identity remains elusive. Here we identify the metalloprotease SPRTN as the DPC protease acting in metazoans. Loss of SPRTN results in failure to repair DPCs and hypersensitivity to DPC-inducing agents. SPRTN accomplishes DPC processing through a unique DNA-induced protease activity, which is controlled by several sophisticated regulatory mechanisms. Cellular, biochemical, and structural studies define a DNA switch triggering its protease activity, a ubiquitin switch controlling SPRTN chromatin accessibility, and regulatory autocatalytic cleavage. Our data also provide a molecular explanation on how SPRTN deficiency causes the premature aging and cancer predisposition disorder Ruijs-Aalfs syndrome.

PubMed: 27871365
DOI: 10.1016/j.molcel.2016.09.031

Experimental method

X-RAY DIFFRACTION (1.75 Å)