5LLY

Photosensory Module of Bacteriophytochrome linked Diguanylyl Cyclase from Idiomarina species A28L

5LLY の概要

• エントリーDOI: 10.2210/pdb5lly/pdb
• 分子名称: Diguanylate cyclase (GGDEF) domain-containing protein, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: bacteriophytochrome, diguanylate cyclase, light-regulation, c-di-gmp, hydrolase, signaling protein
• 由来する生物種: Idiomarina sp. A28L
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 243354.07

構造登録者

Gourinchas, G.,Winkler, A. (登録日: 2016-07-28, 公開日: 2017-03-15, 最終更新日: 2024-10-23)

主引用文献

Gourinchas, G.,Etzl, S.,Gobl, C.,Vide, U.,Madl, T.,Winkler, A.
Long-range allosteric signaling in red light-regulated diguanylyl cyclases.
Sci Adv, 3:e1602498-e1602498, 2017

PubMed Abstract: Nature has evolved an astonishingly modular architecture of covalently linked protein domains with diverse functionalities to enable complex cellular networks that are critical for cell survival. The coupling of sensory modules with enzymatic effectors allows direct allosteric regulation of cellular signaling molecules in response to diverse stimuli. We present molecular details of red light-sensing bacteriophytochromes linked to cyclic dimeric guanosine monophosphate-producing diguanylyl cyclases. Elucidation of the first crystal structure of a full-length phytochrome with its enzymatic effector, in combination with the characterization of light-induced changes in conformational dynamics, reveals how allosteric light regulation is fine-tuned by the architecture and composition of the coiled-coil sensor-effector linker and also the central helical spine. We anticipate that consideration of molecular principles of sensor-effector coupling, going beyond the length of the characteristic linker, and the appreciation of dynamically driven allostery will open up new directions for the design of novel red light-regulated optogenetic tools.

PubMed: 28275738
DOI: 10.1126/sciadv.1602498

実験手法

X-RAY DIFFRACTION (2.4 Å)