5LIN

Lysozyme, collected at rotation 1 degree per second

5LIN の概要

• エントリーDOI: 10.2210/pdb5lin/pdb
• 分子名称: Lysozyme C, CHLORIDE ION (3 entities in total)
• 機能のキーワード: lysozyme, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14402.07

構造登録者

Casanas, A.,Finke, A.,Wang, M. (登録日: 2016-07-15, 公開日: 2016-08-03, 最終更新日: 2024-11-06)

主引用文献

Casanas, A.,Warshamanage, R.,Finke, A.D.,Panepucci, E.,Olieric, V.,Noll, A.,Tampe, R.,Brandstetter, S.,Forster, A.,Mueller, M.,Schulze-Briese, C.,Bunk, O.,Wang, M.
EIGER detector: application in macromolecular crystallography.
Acta Crystallogr D Struct Biol, 72:1036-1048, 2016

PubMed Abstract: The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine ϕ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.

PubMed: 27599736
DOI: 10.1107/S2059798316012304

実験手法

X-RAY DIFFRACTION (1.496 Å)