5LFX

Lambda-[Ru(phen)2(dppz-11,12-Me)]2+ bound to a short substituted DNA sequence

5LFX の概要

• エントリーDOI: 10.2210/pdb5lfx/pdb
• 分子名称: DNA (5'-D(*GP*CP*CP*G)-3'), DNA (5'-D(*(CBR)P*GP*GP*C)-3'), BARIUM ION, ... (5 entities in total)
• 機能のキーワード: dna, ruthenium, light-switch, intercalation
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3291.71

構造登録者

Hall, J.P.,Cardin, C.J. (登録日: 2016-07-04, 公開日: 2017-02-15, 最終更新日: 2024-05-08)

主引用文献

Hall, J.P.,Gurung, S.P.,Henle, J.,Poidl, P.,Andersson, J.,Lincoln, P.,Winter, G.,Sorensen, T.,Cardin, D.J.,Brazier, J.A.,Cardin, C.J.
Guanine Can Direct Binding Specificity of Ru-dipyridophenazine (dppz) Complexes to DNA through Steric Effects.
Chemistry, 23:4981-4985, 2017

PubMed Abstract: X-ray crystal structures of three Λ-[Ru(L) dppz] complexes (dppz=dipyridophenazine; L=1,10-phenanthroline (phen), 2,2'-bipyridine (bpy)) bound to d((5BrC)GGC/GCCG) showed the compounds intercalated at a 5'-CG-3' step. The compounds bind through canted intercalation, with the binding angle determined by the guanine NH group, in contrast to symmetrical intercalation previously observed at 5'-TA-3' sites. This result suggests that canted intercalation is preferred at 5'-CG-3' sites even though the site itself is symmetrical, and we hypothesise that symmetrical intercalation in a 5'-CG-3' step could give rise to a longer luminescence lifetime than canted intercalation.

PubMed: 28105682
DOI: 10.1002/chem.201605508

実験手法

X-RAY DIFFRACTION (1.56 Å)