5LFW

Lambda-[Ru(phen)2(dppz)]2+ bound to a short substituted DNA sequence

Summary for 5LFW

• Entry DOI: 10.2210/pdb5lfw/pdb
• Descriptor: DNA (5'-D(*GP*CP*CP*G)-3'), DNA (5'-D(*(CBR)P*GP*GP*C)-3'), BARIUM ION, ... (5 entities in total)
• Functional Keywords: dna, ruthenium, intercalation, light-switch
• Biological source: synthetic construct; More
• Total number of polymer chains: 2
• Total formula weight: 3263.66

Authors

Hall, J.P.,Cardin, C.J. (deposition date: 2016-07-04, release date: 2017-02-15, Last modification date: 2024-05-08)

Primary citation

Hall, J.P.,Gurung, S.P.,Henle, J.,Poidl, P.,Andersson, J.,Lincoln, P.,Winter, G.,Sorensen, T.,Cardin, D.J.,Brazier, J.A.,Cardin, C.J.
Guanine Can Direct Binding Specificity of Ru-dipyridophenazine (dppz) Complexes to DNA through Steric Effects.
Chemistry, 23:4981-4985, 2017

PubMed Abstract: X-ray crystal structures of three Λ-[Ru(L) dppz] complexes (dppz=dipyridophenazine; L=1,10-phenanthroline (phen), 2,2'-bipyridine (bpy)) bound to d((5BrC)GGC/GCCG) showed the compounds intercalated at a 5'-CG-3' step. The compounds bind through canted intercalation, with the binding angle determined by the guanine NH group, in contrast to symmetrical intercalation previously observed at 5'-TA-3' sites. This result suggests that canted intercalation is preferred at 5'-CG-3' sites even though the site itself is symmetrical, and we hypothesise that symmetrical intercalation in a 5'-CG-3' step could give rise to a longer luminescence lifetime than canted intercalation.

PubMed: 28105682
DOI: 10.1002/chem.201605508

Experimental method

X-RAY DIFFRACTION (1.28 Å)