5LDS

Structure of the porcine aminopeptidase N ectodomain

Summary for 5LDS

• Entry DOI: 10.2210/pdb5lds/pdb
• Descriptor: Aminopeptidase N, beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-6)-[beta-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• Functional Keywords: cd13, papn, aminopeptidase, coronavirus, receptor, enzime, hydrolase
• Biological source: Sus scrofa (Pig); More
• Total number of polymer chains: 4
• Total formula weight: 440533.09

Authors

Santiago, C.,Reguera, J.,Mudgal, G.,Casasnovas, J.M. (deposition date: 2016-06-27, release date: 2017-04-05, Last modification date: 2024-10-23)

Primary citation

Santiago, C.,Mudgal, G.,Reguera, J.,Recacha, R.,Albrecht, S.,Enjuanes, L.,Casasnovas, J.M.
Allosteric inhibition of aminopeptidase N functions related to tumor growth and virus infection.
Sci Rep, 7:46045-46045, 2017

PubMed Abstract: Cell surface aminopeptidase N (APN) is a membrane-bound ectoenzyme that hydrolyzes proteins and peptides and regulates numerous cell functions. APN participates in tumor cell expansion and motility, and is a target for cancer therapies. Small drugs that bind to the APN active site inhibit catalysis and suppress tumor growth. APN is also a major cell entry receptor for coronavirus, which binds to a region distant from the active site. Three crystal structures that we determined of human and pig APN ectodomains defined the dynamic conformation of the protein. These structures offered snapshots of closed, intermediate and open APN, which represent distinct functional states. Coronavirus envelope proteins specifically recognized the open APN form, prevented ectodomain progression to the closed form and substrate hydrolysis. In addition, drugs that bind the active site inhibited both coronavirus binding to cell surface APN and infection; the drugs probably hindered APN transition to the virus-specific open form. We conclude that allosteric inhibition of APN functions occurs by ligand suppression of ectodomain motions necessary for catalysis and virus cell entry, as validated by locking APN with disulfides. Blocking APN dynamics can thus be a valuable approach to development of drugs that target this ectoenzyme.

PubMed: 28393915
DOI: 10.1038/srep46045

Experimental method

X-RAY DIFFRACTION (2 Å)