5LBD

Crystal structure of the N-domain of HMA6, a copper-transporting P-type ATPase

5LBD の概要

• エントリーDOI: 10.2210/pdb5lbd/pdb
• 分子名称: Copper-transporting ATPase PAA1, chloroplastic, IODIDE ION, AMMONIUM ION, ... (6 entities in total)
• 機能のキーワード: p-type atpase, copper transporter, chloroplast, inner membrane, hydrolase, membrane protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• 細胞内の位置: Plastid, chloroplast membrane ; Multi- pass membrane protein : Q9SZC9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29042.73

構造登録者

Mayerhofer, H.,Ravaud, S.,Pebay-Peyroula, E. (登録日: 2016-06-15, 公開日: 2017-08-02, 最終更新日: 2024-10-16)

主引用文献

Mayerhofer, H.,Sautron, E.,Rolland, N.,Catty, P.,Seigneurin-Berny, D.,Pebay-Peyroula, E.,Ravaud, S.
Structural Insights into the Nucleotide-Binding Domains of the P1B-type ATPases HMA6 and HMA8 from Arabidopsis thaliana.
PLoS ONE, 11:e0165666-e0165666, 2016

PubMed Abstract: Copper is a crucial ion in cells, but needs to be closely controlled due to its toxic potential and ability to catalyse the formation of radicals. In chloroplasts, an important step for the proper functioning of the photosynthetic electron transfer chain is the delivery of copper to plastocyanin in the thylakoid lumen. The main route for copper transport to the thylakoid lumen is driven by two PIB-type ATPases, Heavy Metal ATPase 6 (HMA6) and HMA8, located in the inner membrane of the chloroplast envelope and in the thylakoid membrane, respectively. Here, the crystal structures of the nucleotide binding domain of HMA6 and HMA8 from Arabidopsis thaliana are reported at 1.5Å and 1.75Å resolution, respectively, providing the first structural information on plants Cu+-ATPases. The structures reveal a compact domain, with two short helices on both sides of a twisted beta-sheet. A double mutant, aiding in the crystallization, provides a new crystal contact, but also avoids an internal clash highlighting the benefits of construct modifications. Finally, the histidine in the HP motif of the isolated domains, unable to bind ATP, shows a side chain conformation distinct from nucleotide bound structures.

PubMed: 27802305
DOI: 10.1371/journal.pone.0165666

実験手法

X-RAY DIFFRACTION (1.5 Å)