5L7V

Crystal Structure of BvGH123 with bond transition state analog Galthiazoline.

5L7V の概要

• エントリーDOI: 10.2210/pdb5l7v/pdb
• 分子名称: glycoside hydrolase, (3aR,5R,6R,7R,7aR)-5-(hydroxymethyl)-2-methyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol (3 entities in total)
• 機能のキーワード: tim barrel, bacon, hydrolase
• 由来する生物種: Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 129403.02

構造登録者

Roth, C.,Petricevic, M.,John, A.,Goddard-Borger, E.D.,Davies, G.J.,Williams, S.J. (登録日: 2016-06-03, 公開日: 2017-03-22, 最終更新日: 2024-11-06)

主引用文献

Roth, C.,Petricevic, M.,John, A.,Goddard-Borger, E.D.,Davies, G.J.,Williams, S.J.
Structural and mechanistic insights into a Bacteroides vulgatus retaining N-acetyl-beta-galactosaminidase that uses neighbouring group participation.
Chem. Commun. (Camb.), 52:11096-11099, 2016

PubMed Abstract: Bacteroides vulgatus is a member of the human microbiota whose abundance is increased in patients with Crohn's disease. We show that a B. vulgatus glycoside hydrolase from the carbohydrate active enzyme family GH123, BvGH123, is an N-acetyl-β-galactosaminidase that acts with retention of stereochemistry, and, through a 3-D structure in complex with Gal-thiazoline, provide evidence in support of a neighbouring group participation mechanism.

PubMed: 27546776
DOI: 10.1039/c6cc04649e

実験手法

X-RAY DIFFRACTION (2.3 Å)