5L7A

The crystal structure of the Human SNF5/INI1 domain

5L7A の概要

• エントリーDOI: 10.2210/pdb5l7a/pdb
• 分子名称: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (2 entities in total)
• 機能のキーワード: snf5 ini1 domain crystal, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 32544.89

構造登録者

Allen, M.D.,Zinzalla, G.,Bycroft, M. (登録日: 2016-06-02, 公開日: 2017-05-10, 最終更新日: 2024-01-10)

主引用文献

Sammak, S.,Allen, M.D.,Hamdani, N.,Bycroft, M.,Zinzalla, G.
The structure of INI1/hSNF5 RPT1 and its interactions with the c-MYC:MAX heterodimer provide insights into the interplay between MYC and the SWI/SNF chromatin remodeling complex.
FEBS J., 285:4165-4180, 2018

PubMed Abstract: c-MYC and the SWI/SNF chromatin remodeling complex act as master regulators of transcription, and play a key role in human cancer. Although they are known to interact, the molecular details of their interaction are lacking. We have determined the structure of the RPT1 region of the INI1/hSNF5/BAF47/SMARCB1 subunit of the SWI/SNF complex that acts as a c-MYC-binding domain, and have localized the interaction regions on both INI1 and on the c-MYC:MAX heterodimer. c-MYC interacts with a highly conserved groove on INI1, while INI1 binds to the c-MYC helix-loop-helix region. The binding site overlaps with the c-MYC DNA-binding region, and we show that binding of INI1 and E-box DNA to c-MYC:MAX are mutually exclusive.

PubMed: 30222246
DOI: 10.1111/febs.14660

実験手法

X-RAY DIFFRACTION (2.102 Å)