5L73

MAM domain of human neuropilin-1

5L73 の概要

• エントリーDOI: 10.2210/pdb5l73/pdb
• 分子名称: Neuropilin-1, CALCIUM ION, BICINE, ... (5 entities in total)
• 機能のキーワード: mam domain, dimerisation domain, neuropilin, signaling protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted: O14786
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44736.79

構造登録者

Yelland, T.,Djordjevic, S. (登録日: 2016-06-01, 公開日: 2016-10-26, 最終更新日: 2024-10-16)

主引用文献

Yelland, T.,Djordjevic, S.
Crystal Structure of the Neuropilin-1 MAM Domain: Completing the Neuropilin-1 Ectodomain Picture.
Structure, 24:2008-2015, 2016

PubMed Abstract: Neuropilins (NRPs) are single-pass transmembrane receptors involved in several signaling pathways that regulate key physiological processes such as vascular morphogenesis and axon guidance. The MAM domain of NRP, which has previously been implicated in receptor multimerization, was the only portion of the ectopic domain of the NRPs for which the structure, until now, has been elusive. Using site-directed mutagenesis in the linker region preceding the MAM domain we generated a protein construct amenable to crystallization. Here we present the crystal structure of the MAM domain of human NRP1 at 2.24 Å resolution. The protein exhibits a jellyroll topology, with Ca ions bound at the inter-strand space enhancing the thermostability of the domain. We show that the MAM domain of NRP1 is monomeric in solution and insufficient to drive receptor dimerization, which leads us to propose a different role for this domain in the context of NRP membrane assembly and signaling.

PubMed: 27720589
DOI: 10.1016/j.str.2016.08.017

実験手法

X-RAY DIFFRACTION (2.24 Å)