5L24

Structure of CNTnw N149L in the intermediate 2 state

Summary for 5L24

• Entry DOI: 10.2210/pdb5l24/pdb
• Related: 5L26 5L27 5L28 5L29 5L2A 5L2B
• Descriptor: Nucleoside permease, 2-{[(4-O-alpha-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]methyl}-2-octyldecyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside (2 entities in total)
• Functional Keywords: transporter, nucleoside, elevator-type alternate access, transport protein
• Biological source: Neisseria wadsworthii 9715
• Total number of polymer chains: 3
• Total formula weight: 135918.97

Authors

Hirschi, M.,Johnson, Z.L.,Lee, S.-Y. (deposition date: 2016-07-31, release date: 2017-04-12, Last modification date: 2023-10-04)

Primary citation

Hirschi, M.,Johnson, Z.L.,Lee, S.Y.
Visualizing multistep elevator-like transitions of a nucleoside transporter.
Nature, 545:66-70, 2017

PubMed Abstract: Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion.

PubMed: 28424521
DOI: 10.1038/nature22057

Experimental method

X-RAY DIFFRACTION (4.1 Å)